Antimicrobial activity of some plant extracts against. Preliminary phytochemical analysis and antimicrobial activity. Moreover, antimicrobial activity of different natural substances such as medicinal plant extract have been investigated against food borne bacteria. Antimicrobial coating of cotton twill tape with neem oil. In present study, four important commonly used indian medicinal plants azadiracta indica neem, cymbopogon citratus lemon grass, mentha arvenis pudina and ocimum sanctum tulsi were selected and antibacterial activity of their leaf extracts was evaluated against some bacterial pathogens. Evaluation of the antimicrobial activity of various. Study of antimicrobial properties of fabrics treated with ocimum. Tulsidas was a hindu poetsaint counted amongst the greatest poets in hindi, indian, and world literature. Antimicrobial activity of tulsi leaves extract was evaluated and the results shown that. Invitro comparative study of antibacterial and antifungal activities. Choudhury et al 10 studied pharmacognostical and phytochemical screening of various tulsi plants available in south eastern odisha state of india.
Antibacterial effect of herbs and spices extract on escherichia coli venugopal amrita, dasani sonal, rai shalini department of life sciences, kishinchand chellaram college, d. The 500 mg l1 of extract treated water showed 95 to 98% antibacterial activity in 14 to 16 hrs. Essential oils extracted from the leaves of ocimum sanctum l. Review article chemical constituents and pharmacological. Purpleleafed tulsi, also known as krishna or shyama tulsi, has a sharp peppery taste and is harder to find than other varieties. The aims of this project were to examine the antimicrobial properties of essential oils distilled from australiangrown ocimum tenuiflorum tulsi. Ocimum sanctum also possesses antifungal activity against asperigillus niger and aqueous extract of it was found to be effective in patients suffering from viral encephalitis. Antimicrobial activity of neem, clove, curry leaves. To check the antimicrobial activity of azadirachta indica neem, ocimum sanctum tulsi, mimusops elelngi bakul, tinospora cardifolia giloy and chlorhexidine gluconate chx on common endodontic pathogens like streptococcus mutans, enterococcus faecalis and staphylococcus aureus. Pdf antimicrobial activity of tulsi ocimum tenuiflorum essential. Pdf comparative study on antimicrobial activity of tulsi. Pdf antimicrobial and enzyme activity of endophytic fungi.
Antimicrobial efficacy of tulsi leaf ocimum sanctum extract. Bandita deo 1, monalisha nath2, preetam kumar nayak 3, yogamaya dhal4 regional plant resource centre, nayapali, bhubaneswar, odisha, india. The clinical efficacy and safety of tulsi in humans. Ursolic acid is responsible for its antisterility property 1. Individual suspensions of microorganisms were added. Antibacterial activity of aqueous, ethanol and acetone. In this, 600 mg l1 concentration of plant extract treated water showed effective antimicrobial activity at 15 to 16 hrs than the other concentration of extract. It is similarly beneficial in soothing asthmatic reactions. Extracts of neem and tulsi demonstrated an antimicrobial activity but triphla has been failed to show antimicrobial activity against s.
Review article welcome to your tulsi wellness cleanse community. The aims of this project were to examine the antimicrobial properties of essential oils distilled from australiangrown ocimum tenuiflorum tulsi, to quantify the. Its subdisciplines are virology study of viruses, bacteriology study of bacteria. Antimicrobial activity of tulsi ocimum tenuiflorum essential oil and. Antimicrobial activity refers to the ability of a substance to reduce or inhibit the growth of microorganisms. Studies on antimicrobial textile finish using certain plant. The aims of this project were to examine the antimicrobial properties of essential oils distilled from australiangrown ocimum tenuiflorum tulsi, to quantify the volatile components present in flower spikes, leaves and the essential oil, and to investigate the compounds responsible for any activity.
Medicinal properties of tulsi ocimum sanctum linn are known. Studies on antimicrobial textile finish using certain plant natural products s. Antimicrobial studies indicated that both the acetone leaf and root extracts of o. Rama tulsi, or green leaf tulsi, has a mild taste with a strong aroma. Singh et al biological activities of ocimum sanctum l. Antimicrobial activity of neem, tulsi, henna and amla against. Antimicrobial activity of plant extracts against post harvest spoilage of onions v. Evaluation of the antiinflammatory activity of ocimum. Rathod et al 17 have reported antimicrobial activity of aqueous and ethanolic extract of neem leaves and bark and tulsi leaves against two gram positive bacteria b. Accepted 27 september 2005 abstract tulsi ocimum sanctum linn. Leaf extract of 37 plants were tested against xcmi. Evaluation of some chemical composition, minerals fatty.
Sanctum against known bacterial with contact time 30 minutes under invitro conditions tulsi o. Biosynthesis of silver nanoparticles using ocimum sanctum. The chemical composition of tulsi is highly complex, containing many nutrients and other biological active compounds. Leaf and bark extract of azadirachta indica showed more inhibition zone against vibrio cholerae and bacillus subtilis, while e. The fact that a plant extract exhibits antimicrobial activity is of interest, but this preliminary part of data should be trustworthy and allow researchers to compare results, avoiding work in which researchers use the antimicrobial activity investigation only as a complement to a phytochemical study. Antimicrobial activities of alcoholic extracts of neem leaves were used. The former possesses greater medicinal value and is commonly used for worship. The results of the study suggest that endophytic fungi associated with tulsi are potential agents for antimicrobial activity and production of enzyme. Stability of bioreduced silver nanoparticles was analyzed using uvvis absorption spectra, and their antimicrobial activity was screened against both gramnegative and grampositive microorganisms. Studies of antimicrobial properties of different leaf. Antimicrobial activity of tulsi ocimum tenuiflorum.
Synthesis of silver nanoparticles from the aqueous extract. Immunomodulatory effects of tulsi ocimum sanctum linn. Phytochemical analysis, antibacterial and antioxidant activity determination of ocimum sanctum. Significant antimicrobial activity of plant extract has been observed. Aqueous, ethanol and acetone leaf and root extracts of ocimum sanctum linn were investigated for their antibacterial activities at various concentrations against escherichia coli, staphylococcus aureus and pseudomonas aeruginosa using standard methods. Of the many varieties, the krishna or shyama tulsi is also commonly used for worship. The extracts exhibited antibacterial activity at concentrations of 25 and 10 mgml with mbc of both extracts ranged between 3. Fixed oil 405 prostaglandin synthesis could help to explain their antipyretic activity12. Tulsi oil loaded biocompatible, stable organogel with.
Antimicrobial activity of various extract of leaves of ocimum tenuiflorum was carried in attempt to develop a new pharmaceutical drug from natural origin for prevention of pathogenic microbes. He was great poet in hindi, indian, world literature and founded the temple sankat mochan. Studies of antimicrobial properties of different leaf extract of tulsi. After the first wash it was seen that inhibition persisted against all species. Therapeutic potential and phytopharmacology of tulsi. In males, tulsi leaves reduce spermatogenesis by retarding sertoli cells activity 28. Evaluation and comparison of antimicrobial activity of. Tulsi has environmental benefits too times of india. The present study deals with the antimicrobial activity of different samples like neem, clove, curry leaves, cardamom, tulsi stem and tulsi leaves. Scholars research library phytochemical estimation and. Antimicrobial activity, ocimum sanctum, streptococcus mutans, tulsi extract. Pdf antimicrobial activity of ocimum sanctum leaves extracts and oil. Assessment of antimicrobial activity of various concentrations of commercially available tulsi ocimum sanctum powder against streptococcus mutans. Varying concentration of each extracts 200mgml, 150 mgml, 100mgml.
Jul 17, 2017 several varieties of holy basil grow throughout the himalayan foothills to areas of the middle east. Tulsi named as queen of herbs of india and one of the holiest and most healthiness giving herbs. Tulsi leaves have antiandrogenic property 29 as well. Formulation and evaluation of naproxen gel containing tulsi. Medicinal properties, ocimum sanctum os, tulsi, pharmacological activities. Introduction tulsi is an important symbol of the hindu religious tradition. Thus, this method can be used for rapid and ecofriendly biosynthesis of stable silver nanoparticles of size range 430 nm possessing antimicrobial activity suggesting their possible application in medical industry. Review article pharmacological activities of ocimum sanctum. Antimicrobial effects of ocimum sanctum on oral microbes. Antioxidant activity and phenolic contents in ocimum sanctum. Antimicrobial activity antibacterial activity introduction the science dealing with the study of the prevention and treatment of diseases caused by microorganisms is known as medical microbiology. In their preliminary study, although tulsi did not show any activity, it exhibited a bacterial reduction of 73% in challenge test. The antimicrobial activity of azadirachta indica, mimusops. Tulsi, also known as holy basil, is indigenous to the indian continent and highly revered for its medicinal uses within the ayurvedic and siddha medical systems.
The agar diffusion method well or paper disk and the dilution method liquid broth or agar are the two basic techniques used for the examination of the antimicrobial activity of the essential oils. Of these, the agar diffusion method was commonly used in earlier studies. Antimicrobial activity of tulsi ocimum tenuiflorum essential oil and their major constituents against three species of bacteria yamani, h, pang, e, mantri, n and deighton, m 2016, antimicrobial activity of tulsi ocimum tenuiflorum essential oil and their major constituents against three species of bacteria, frontiers in microbiology, vol. Antibacterial assessment antibacterial activity of the extracts was evaluated by using paper disc agar diffusion method. Most of these evidences are based on invitro, experimental and a few human studies. Ocimum sanctum, known as tulsi in hindi and holy basil in english, is an erect softy hairy aromatic herb or undershurb found throughout india. Thus, a varied range of natural products of plant, fungi and lichen origin were tested against two bacterial species, staphylococcus aureus atcc 25923 and escherichia coli atcc 25922, by. International journal of pharmtech research coden usa. A holy plant with high medicinal and therapeutic value. We report the synthesis of antibacterial silver nanoparticles agnps using leaf broth of medicinal herb, ocimum sanctum tulsi. Tulsi on immune system, central nervous system, gastric system, reproductive system, blood biochemistry etc.
Antimicrobial activity of tulsi ocimum tenuiflorum essential oil and their major constituents against three species of bacteria. The present study focuses on the phytochemical analysis, antioxidant activity and antimicrobial activity of cyathocline lyrata leaf collected from melghat forest talchikhaldara, distamravati. Many in vitro, animal and human studies attest to tulsi having multiple therapeutic actions including adaptogenic, antimicrobial, antiinflammatory, cardioprotective, and immunomodulatory effects, yet to date there are no systematic. Study has been shownthe presence of steroids, alkaloids and tannins. Antioxidant activity and phenolic contents in ocimum sanctum and ocimum bascilicum inampudi sailaja1, ivvala anand shaker2 and yedlapalli keerthi ratna1 1department of biochemistry and molecular biology, college of agricultural biotechnology, loni,ahmednagar, m. Antibacterial activity of garlic, tulsi, bitter guard and. The leaves, seeds and root of this plant have been used in indigenous ayurvedic medicine. Tulsi gives out oxygen for 20 hours and ozone for four hours a day along with the formation of nascent oxygen which.
Assessment of antimicrobial activity of various concentrations of. Tulsi is famous for its vital role in the conventional ayurvedic and unani. Mahapatra1 departments of 1physiology and 2microbiology, all india institute of medical sciences, new delhi 110 029 received on june 18, 2009 abstract. Pdf the aim of this study was to evaluate the antimicrobial effects of ethanol. The fixed oil of tulsi ocimum sanctum is reported to possess significant anti inflammatory activity. Combined antibacterial activity of stingless bee apis on garlic, tulsi and cinnamon which ranged between 0. Eugenol and linalool have been investigated against two species of candida i.
Antibacterial activity of leaf extracts of ocimum sanctum. Antimicrobial activity, phytochemical analysis, ocimum tenuiflorum. Evaluation of the antimicrobial activity of various concentrations of tulsi ocimum sanctum extract against streptococcus mutans. Studies of antimicrobial properties of different leaf extract. Review article welcome to your tulsi wellness cleanse. Two type of ocimum sanctum are met within cultivation i. Naproxen gels containing various concentration of tulsi oil 1 %, 3 %, 5 %, and 7 % were prepared. Pdf antimicrobial activity of ocimum sanctum leaves. India 2, pharmacology division, department of pharmaceutical sciences, guru jambeshwar university of science and technology, hisar, haryana india abstract. To study the antimicrobial activity of the different leaf extracts of tulsi ocimum tenuiflorum, also known as ocimum sanctum, against three human. Its diaphoretic properties are sought especially in cases of malarial fever in the form of a root decoction 4. Medicinal uses of tulsi tulsi has innumerable medicinal applications ranging from treating mundane coughs and colds effectively to rendering poisoning inactive. Various studies conducted to study and establish the pharmacological properties of tulsi show that it has numerous pharmacological properties, many of which, like radioprotection and anticancer, can prove to be very important for the human race in this age of industrialization.
Escherichia coli, proteus mirabilis, staphylococcus aures. Although the medical usefulness of tulsi is investigated extensively, its effectiveness in treating oral diseases is not yet established, and only a few studies are available in the literature regarding this aspect. Mar 05, 2015 when tulsi gabbard first ran for congress in 2012 from her home state of hawaii, her republican opponent, david kawika crowley, ridiculed the observant hindu for subscribing to a religion. Dy patil dental college hospital, pune, maharashtra, india 2department of public health dentistry, vyas dental college, jodhpur, rajasthan, india. Antimicrobial activity of neem, tulsi, henna and amla against pathogenic bacteria seema rawat department of botany and microbiology, h. Though there are many chemical as well as physical methods, green synthesis of nanomaterials is the most emerging method of synthesis. Phytochemical analysis and antibacterial properties of. Antimicrobial finishing of denim fabrics with herbal extracts. The mbc results showed that tulsi essential oil had only bacteriostatic. Tulsi is branched shrub 3060 cm tall with hairy stem. Review article pharmacological activities of ocimum. The mbc results showed that tulsi essential oil had only bacteriostatic activity against the examined bacterial strains.
However, literature pertaining to the antimicrobial activity of tulsi on periodontal bacteria has not been reported earlier. Antimicrobial activity of neem, tulsi, henna and amla. Phytochemical analysis, antibacterial and antioxidant. Antimicrobial activity of neem, clove, curry leaves, cardamom. Natesh3 1department of agricultural microbiology, uas, gkvk, bangalore65, india 3department of seed science and technology, uas, gkvk, bangalore 65, india corresponding author abstract introduction.
Materials and methods collection of samples the medicinal plants used for the experiment were leaves of ocimum sanctum tulsi, flower of eugenia caryophyllata clove. Antimicrobial activity of plant extracts against post harvest. Page 1 queen of herbs tulsi ocimum sanctum removes impurities from water and plays disinfectant role babita labh kayastha 1 1. He was the composer of the original ramayana into sanskrit. Tulsi is an incredible herb revered in indian mythology for its medicinal properties. Antibacterial activity of garlic, tulsi, bitter guard and cinnamon extracts against wound pathogens godfrey omare mauti, eliakim mbaka mauti, geoffrey arasa ouno, benard maronga mabeya in the present study antibacterial properties of fruits harvested from natural medicinal plants garlic, tulsi, bitter. Pdf antibacterial activity of garlic, tulsi, bitter. Phytochemical extraction and antimicrobial properties of. Materials and methods source of tulsi tulsi ocimum tenui. Antibacterial effect of herbs and spices extract on. The antimicrobial activity of leaf distillate of o. The herb grows wild in tropical and subtropical regions, according to. Jan 18, 2014 the ubiquitous tulsi in your backyard may be a potent weapon against all kinds of cancer, so believes a team of researchers led by an indianorigin scientist.
Comparison with conventional soxhlet extraction method rahimah bt abd rahim report submitted in partial fulfillment of the requirements for the award of the degree of bachelor of chemical engineering. Antibacterial activity antibacterial activity of the aqueous, alcoholic, chloroform extract and oil obtained from leaves of ocimum sanctum were studied against li, p. In india two forms of tulsi are more common dark or shyama krishna tulsi and light or rama tulsi. The field of nanotechnology is the most active area of research in modern materials science. May 17, 2016 the antimicrobial activity of tulsi essential oil.
Although the word tulsi gives the connotation of the incomparable one, its other name, vishnupriya means the one that pleases lord vishnu. Phytoconstituents, traditional, medicinal and bioactive uses of tulsi. Therefore, in the current study, the antimicrobial activity of alcoholic extract of tulsi. Antibacterial activity of leaf extracts of aloe vera. Antimicrobial activity of tulsi leaves extract was evaluated and the results shown that e. Vijaya kumar r international conference on advances in biotechnology and pharmaceutical sciences icabps2011 bangkok dec. Therapeutic potential and phytopharmacology of tulsi renu kadian 1 and milind parle 2 1, iimt college of pharmacy, greater noida, u. Antimicrobial activity in leaf extract of neem azadirachta indica against human pathogenic bacteria. Evaluation of the antiinflammatory activity of ocimum sanctum linn tulsi in albino rats mrutyunjay m mirje1, sameer uz zaman2, and s.
Tulsi, as many diaphoretics, is also used commonly as a response to skin disease and itch 2. An in vitro study pooja agarwal 1, l nagesh 1, murlikrishnan 2 1 department of preventive and community dentistry, bapuji dental college and hospital, davangere 577 004, karnataka, india 2 department of. Ramabhimaiah3 1dept of pharmacology, institute of medical science and research, mayni, tal katva, satara415102, india 2kamineni academy of medical science and research centre, l. Methods for in vitro evaluating antimicrobial activity. Different substances have different extent of antimicrobial activity.
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